文献名： Electrospinning fabrication of covalent organic framework composite nanofibers for pipette tip solid phase extraction of tetracycline antibiotics in grass carp and duck

作者： Ronglin Wanga; Canru Lia; Qianlian Lia; Sunxian Zhangb; Feng lva; Zhiming Yana

aCollge of Food Science, Fujian Agriculture and Forestry University, 350002, Fuzhou, Fujian, China

bFujian Inspection and Research Institute for Product Quality, 350001, Fuzhou, Fujian, China

摘要：In the current study, a novel covalent organic frameworks COF-SCU1 incorporated electrospun nanofibers (PAN@COF-SCU1 nanofibers) was fabricated via a facile electrospinning method and utilized as adsorbent in pipette tip solid-phase extraction (PT-SPE) of tetracycline antibiotics (TCs) from foods. The prepared PAN@COF-SCU1 nanofibers possessed both of the unique characteristics of electrospun nanofibers and COFS-CU1, and thus improving the adsorption capacity of the electrospun nanofibers and preventing the problems of leakage and high pressure caused by directly using the nanosize COFs as adsorbent in PT-SPE. The experiments affected the adsorption and desorption efficiencies, such as the loading ratios of COFS-CU1 in nanofibers, the amount of nanofibers, the matrix pH and desorption solvent, were studied in detail. Eventually, a new pipette tip solid-phase extraction-high performance liquid chromatography (PT-SPE/HPLC) method was proposed for the analysis of three TCs from food. Satisfied linearity for TCs was obtained in the range of 4–70 ng mL−1. The limits of detection and quantification were ranged from 0.6 to 3 ng mL−1 and from 2 to 10 ng mL−1, respectively. The interday and intraday precisions (RSD) were all lower than 9%. The proposed PT-SPE/HPLC method was used to determine TCs residues in grass carp and duck samples for the first time. The results could not only explore the availability of PT-SPE in the extraction of TCs in food samples, but also broadened the potential applications of COFs in sample preparation.